Environmental education, its connection with practice and education for sustainable development have been frequently criticized in Slovakia in recent years. Since the main goal of biosphere reserves is to effectively use the territory of the reserve as a “living laboratory” for sustainable development and education, we decided to conduct research in one of them and find out how environmental education is carried out in educational settings and to what extent the biosphere reserve is used in the teaching process. The issue of the Tatra Biosphere Reserve (BR) involvement in environmental education is complex and faces several structural, institutional and perceptual challenges. In a questionnaire survey, we addressed 402 environmental institutions operating in the territory of the biosphere reserve. Of these, only 14.21% responded to the survey, mostly kindergartens and primary schools. The topic of biosphere reserves is absent in environmental education, cooperation between the TANAP Administration and educational institutions is weak, and there is no functional educational network in the territory whose members would cooperate with each other. Therefore, we recommend incorporating the topic of biosphere reserves into the formal teaching process.