Dry grasslands in the Protected Landscape Area and Biosphere Reserve of the White Carpathian Mts. in the eastern part of the Czech Republic belong to the most species rich plant communities in Europe. Many of the grasslands were converted to arable land in the second half of the 20th century, but in the past two decades several arable fields have been re-grassed, most of them using commercial clover-grass seed mixtures, some however by spontaneous succession, and since 1999 also by sowing a regional seed mixture. The regional mixture consists of seeds obtained from existing species-rich meadows in two ways: (1) reproduction of collected seed, (2) harvesting with a combine or brush harvester. With this regional seed mixture more than 500 ha of arable land have been transformed into grassland at 35 localities. To investigate the development of the created meadows, their vegetation was analysed between 2000 and 2011. The regional seed composition was confirmed to be appropriate and to enhance biodiversity. It was also found that although the re-grassed sites develop differently (according to re-grassing method), their vegetation always directs towards the original hay meadows.