In secondary pine and spruce forests of the Spiš-Gemer region several chemical soil parameters, biomass of micromycetes and especially the occurrence of soil testacean rhizopods were studied. Studied soils (Dystric Cambisols) were found extremely to ultra acid and extremely unsaturated (Table 1). The biomass of micromycetes, since the samples consisted of forest litter mixed with humus horizon was relatively high, about 380 μg.g–1 in the pine stand, and 280 μg.g–1 in spruce stand. To acid soil conditions reacted the soil microfauna community as well. Species abundance was not high, we noted species such as Trigonopyxis arcula, Cyclopyxis eurystoma, C. kahli and Nebela tincta. Probably species Arcella conica is also present which, however, is not presented in the Data bank of Slovak fauna (Anonymous). A quantitatively higher abundance was found in spruce stands, what is probably related to higher soil moisture.