Forest fire is an important phenomenon affecting forest ecosystem. It causes essential changes in ecological conditions (e.g. light availability, water and nutrient regime). The aim of this paper is to evaluate its impact on soil as well as herb and moss layer of the secondary pine forest. The study area is located in the southwestern part of Slovakia (Borská nížina lowland). Marked changes in physical and chemical soil properties were induced by forest fire. Soils moisture contents were higher in burnt soil probably as a result of increased soil wettability. Infiltration rate decreased on the burnt area due to sealing of pores by ash material. Burning of soil organic matter caused increase in soil pH and available nutrients. In the period of 2005–2007, floristic composition, biomass production and presence/absence of mushrooms were recorded both for burnt and neighboring control areas. Compare to the control stands, especially nitrophilous (synanthropic) species are appearing, first of all Conyza canadensis, Solidago canadensis and Chamerion angustifolium. Also composition of mycoflora is changed – its composition is shifted on the behalf of pioneer and indifferent species. Moreover, the pine natural seeding is obvious here. Information on succession is supported by biomass production estimation.